Human complement factor I(CFI) ELISA Kit

Human complement factor I(CFI) ELISA Kit

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Mfr.No.

CSB-E13108h

Sensitivity

1.56 ng/mL

Detection Range

6.25 ng/mL-400 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

complement factor I, AHUS3, C3BINA, C3b-INA, FI, IF, KAF, C3B/C4B inactivator, C3b-inactivator, Konglutinogen-activating factor, complement component I, complement control protein factor I, complement factor I heavy chain, li

Recovery

The recovery of rat MCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	104	100-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat MCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	90-92
1:2	Average %	92
	Range %	87-96
1:4	Average %	94
	Range %	89-97
1:8	Average %	87
	Range %	82-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.464	2.489	2.477	2.326
20	1.823	1.863	1.843	1.692
10	1.229	1.263	1.246	1.095
5	0.812	0.847	0.830	0.679
2.5	0.482	0.498	0.490	0.339
1.25	0.312	0.339	0.326	0.175
0.625	0.241	0.258	0.250	0.099
0	0.150	0.152	0.151	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P05156

Applications	ELISA
Research Area	CFI

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501929	CSB-E13108h	96T	$595	Add to Cart
BHE10501929	CSB-E13108h	5x96T	$2856	Add to Cart
BHE10501929	CSB-E13108h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.