Human Complement component C8 alpha chain(C8A) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL004194HU

Alternative Names

complement component 8, alpha polypeptide

Reactivity

Human

Detection Range

1.56 ng/mL-100 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human UCN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	99	96-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human UCN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-89
1:2	Average %	101
	Range %	98-104
1:4	Average %	105
	Range %	101-109
1:8	Average %	90
	Range %	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.39 ng/mL

Limit Of Detection

450 nm

UniProt ID	P07357
Antigen	complement component 8, alpha polypeptide

Applications

ELISA

Research Area

C8A

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501920	CSB-EL004194HU	96T	$595	Add to Cart
BHE10501920	CSB-EL004194HU	5x96T	$2856	Add to Cart
BHE10501920	CSB-EL004194HU	10x96T	$5652.50	Add to Cart