Human Complement C1q subcomponent subunit A(C1QA) ELISA kit

Human Complement C1q subcomponent subunit A(C1QA) ELISA kit

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Mfr.No.

CSB-EL003637HU

Alternative Names

complement component 1, q subcomponent, A chain, OTTHUMP00000197085, complement component 1, q subcomponent, alpha polypeptide, complement component C1q, A chain, complement component 1, q subcomponent, A chain, OTTHUMP00000197085, complement component 1, q subcomponent, alpha polypeptide, complement component C1q, A chain

Reactivity

Human

Detection Range

9.38 ng/mL-600 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human PEPD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-103
EDTA plasma (n=4)	92	88-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PEPD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	84
	Range %	80-89
1:10	Average %	88
	Range %	84-92
1:20	Average %	90
	Range %	85-96
1:40	Average %	85
	Range %	80-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
6000	2.652	2.472	2.562	2.413
3000	2.087	1.976	2.032	1.883
1500	1.293	1.204	1.249	1.100
750	0.598	0.561	0.580	0.431
375	0.379	0.357	0.368	0.219
187.5	0.208	0.214	0.211	0.062
93.75	0.165	0.159	0.162	0.013
0	0.153	0.145	0.149	?

Species

Homo sapiens

Sensitivity

2.34 ng/mL

Limit Of Detection

450 nm

UniProt ID	P02745
Antigen	complement component 1, q subcomponent, A chain

Applications

ELISA

Research Area

C1QA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501903	CSB-EL003637HU	96T	$695	Add to Cart
BHE10501903	CSB-EL003637HU	5x96T	$3336	Add to Cart
BHE10501903	CSB-EL003637HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.