Human Complement 3,C3 ELISA Kit

Human Complement 3,C3 ELISA Kit

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Mfr.No.

CSB-E08665h

Alternative Names

Complement 3,C3

Reactivity

Human

Detection Range

4.7 ng/mL-300 ng/mL

Sample Type

Saliva, Serum, Cell culture supernatants, Plasma, Urine

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-99
EDTA plasma (n=4)	99	95-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-95
1:2	Average %	98
	Range %	92-100
1:4	Average %	90
	Range %	87-96
1:8	Average %	91
	Range %	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
100	2.893	3.112	3.003	2.890
50	2.323	2.394	2.359	2.246
25	1.487	1.555	1.521	1.408
12.5	0.902	0.980	0.941	0.828
6.25	0.431	0.408	0.420	0.307
3.12	0.347	0.321	0.334	0.221
1.56	0.248	0.260	0.254	0.141
0	0.115	0.110	0.113

Species

Homo sapiens

Sensitivity

4.031 ng/mL

Limit Of Detection

450 nm

UniProt ID	P01024
Antigen	Complement 3,C3

Applications

ELISA

Research Area

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Proteomic Identification of Biomarkers Associated with Eating Control and Bariatric Surgery Outcomes in Patients with Morbid Obesity Rodríguez-Rivera C, et al,World Journal of Surgery,November 13

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SKU	Supplier No.	Size	Your price
BHE10501896	CSB-E08665h	96T	$495	Add to Cart
BHE10501896	CSB-E08665h	5x96T	$2376	Add to Cart
BHE10501896	CSB-E08665h	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.