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Home / ELISA Kit

Human Collectrin (TMEM27) ELISA Kit

SKU : BHE13702499

TMEM27 encodes a transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. TMEM27 has an N-terminal signal sequence, followed by a noncatalytic extracellular domain, a transmembrane segment, and a cytosolic domain. The extracellular domain contains an O-glycosylation site and 2 N-glycosylation sites. Human TMEM27 shares 47.8% identity with the C-terminal end of ACE2, but it lacks an ACE2-like catalytic domain. Northern blot analysis of rat, mouse, and human tissues detected a transcript of about 1.8 kb exclusively in kidney.

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Mfr.No.	KTE60281
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Collectrin (TMEM27) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM27.
Alternative Names	TMEM27; NX-17; NX17; 0610008J07Rik; collectrin; kidney-specific membrane protein
Detection Method	Colorimetric
Kit Components	Human Collectrin microplate Human Collectrin standard Human Collectrin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Collectrin (TMEM27) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM27. No significant cross-reactivity or interference between Human TMEM27 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMEM27 encodes a transmembrane protein that is important for trafficking amino acid transporters to the apical brush border of proximal tubules. It also plays a role in controlling insulin exocytosis by regulating formation of the SNARE (soluble N-ethylmaleimide-sensitive-factor attachment protein receptor) complex in pancreatic beta cells. TMEM27 has an N-terminal signal sequence, followed by a noncatalytic extracellular domain, a transmembrane segment, and a cytosolic domain. The extracellular domain contains an O-glycosylation site and 2 N-glycosylation sites. Human TMEM27 shares 47.8% identity with the C-terminal end of ACE2, but it lacks an ACE2-like catalytic domain. Northern blot analysis of rat, mouse, and human tissues detected a transcript of about 1.8 kb exclusively in kidney.
Notes	This Human Collectrin (TMEM27) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM27 in samples. An antibody specific for TMEM27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM27 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM27 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	57393

Applications	ELISA
Applications Notes	This Human Collectrin (TMEM27) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM27 in samples. An antibody specific for TMEM27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM27 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM27 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM27 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702499	KTE60281	48 T	$339		Add to Cart
BHE13702499	KTE60281	96 T	$589		Add to Cart
BHE13702499	KTE60281	96 Tx5	$2629		Add to Cart
BHE13702499	KTE60281	96 Tx50	$24569		Add to Cart

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