Human Collectrin(TMEM27) ELISA kit

Mfr.No.

CSB-EL023823HU

Sensitivity

24 pg/mL

Detection Range

56 pg/mL-800 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

transmembrane protein 27, NX-17, NX17, 0610008J07Rik, collectrin, kidney-specific membrane protein

Recovery

The recovery of human IFI35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	99-108
EDTA plasma (n=4)	93	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IFI35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	96-102
1:2	Average %	95
	Range %	89-100
1:4	Average %	94
	Range %	90-98
1:8	Average %	88
	Range %	86-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9HBJ8

Applications	ELISA
Research Area	TMEM27

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Tmem27 is upregulated by vitamin D in INS-1 cells and its serum concentrations are low in patients with autoimmune diabetes Pepaj M et al,Scand J Clin Lab Invest,2014 Apr 3.

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