Human Collectin-12(COLEC12) ELISA kit

Human Collectin-12(COLEC12) ELISA kit

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Mfr.No.

CSB-EL005762HU

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

collectin sub-family member 12, CLP1, NSR2, SCARA4, SRCL, collectin placenta 1, nurse cell scavenger receptor 2, scavenger receptor class A, member 4, scavenger receptor with C-type lectin

Recovery

The recovery of human blca-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	84-90
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human blca-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-91
1:2	Average %	99
	Range %	96-103
1:4	Average %	104
	Range %	101-107
1:8	Average %	95
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.595	2.540	2.568	2.452
20	2.208	2.365	2.287	2.171
10	1.823	1.804	1.814	1.698
5	1.195	1.175	1.185	1.069
2.5	0.698	0.678	0.688	0.572
1.25	0.418	0.432	0.425	0.309
0.625	0.271	0.276	0.274	0.158
0	0.115	0.117	0.116

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q5KU26

Applications	ELISA
Research Area	COLEC12

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501889	CSB-EL005762HU	96T	$695	Add to Cart
BHE10501889	CSB-EL005762HU	5x96T	$3336	Add to Cart
BHE10501889	CSB-EL005762HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.