Human Collagen Type IV(COL IV) ELISA Kit

Human Collagen Type IV(COL IV) ELISA Kit

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Mfr.No.

CSB-E17116h

Sensitivity

15.6 pg/mL

Reactivity

Human

Sample Type

Serum, Cell culture supernatants, Urine, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Collagen Type IV(COL IV), Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV), Collagen Type IV(COL IV), Collagen Type 4(COL 4),_x000D_ Collagen Type IV(COL IV)

Recovery

The recovery of rat CAM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-102
EDTA plasma (n=4)	104	101-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CAM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	88
	Range %	85-91
1:10	Average %	96
	Range %	94-98
1:20	Average %	89
	Range %	87-90
1:40	Average %	104
	Range %	101-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.819	2.836	2.828	2.632
500	2.181	2.101	2.141	1.945
250	1.494	1.475	1.485	1.289
125	0.919	0.935	0.927	0.731
62.5	0.588	0.598	0.593	0.397
31.2	0.402	0.432	0.417	0.221
15.6	0.287	0.275	0.281	0.085
0	0.195	0.197	0.196

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Antigen	Collagen Type Ⅳ(COL Ⅳ)

Applications	ELISA
Research Area	Col IV

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Clinical significance of decoy receptor 3 upregulation in patients with hepatitis B and liver fibrosis Xiaoli Lou.et al,Oncology Letters,May 21

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SKU	Supplier No.	Size	Your price
BHE10501882	CSB-E17116h	96T	$790	Add to Cart
BHE10501882	CSB-E17116h	5x96T	$3792	Add to Cart
BHE10501882	CSB-E17116h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.