Human Collagen Type I,Col I ELISA Kit

Human Collagen Type I,Col I ELISA Kit

Reviews of Human Collagen Type I,Col I ELISA Kit

Rate it now..!!

Rate it now..!!

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Mfr.No.

CSB-E08082h

Sensitivity

0.39 ng/mL

Detection Range

1.56 ng/mL-100 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Collagen Type I,Col I, Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I, Collagen Type I,Col I, Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I

Recovery

The recovery of human BRAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-104
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BRAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	80-100
1:2	Average %	97
	Range %	91-105
1:4	Average %	99
	Range %	92-110
1:8	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.408	2.432	2.420	2.279
1000	1.659	1.676	1.668	1.527
500	1.042	1.004	1.023	0.882
250	0.660	0.639	0.650	0.509
125	0.429	0.424	0.427	0.286
62.5	0.289	0.275	0.282	0.141
31.25	0.214	0.229	0.222	0.081
0	0.145	0.137	0.141	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Col I

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Photoprotective effect of the N-terminal 5-mer peptide analog P165 of amyloid precursor protein in human dermal fibroblasts. Wang Y et al,Chin Med J (Engl),2014 Feb;127(4):718-23. N-terminal 5-mer peptide analog P165 of amyloid precursor protein inhibits UVA-induced MMP-1 expression by suppressing the MAPK pathway in human dermal Wang Y et al,Eur J Pharmacol,2014 Mar 29.pii: S0014-2999(14)00234-9. Chondrogenic Differentiation Processes in Human BoneMarrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I Frisch J. et al,Tissue Eng Part A. ,2015 Sep;21(17-18):2460-71. Chondrogenic Differentiation Processes in Human BoneMarrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I. Frisch J.et al,Tissue Eng Part A. ,Sep;21(17-18):2460-71 Protective effect of low dose gadolinium chloride against isoproterenol-induced myocardial injury in rat Zheng YY. et al,Apoptosis.,2015 Sep;20(9):1164-75 Enhanced osteoblast differentiation and osseointegration of a bio-inspired HA nanorod patterned pore-sealed MgO bilayer coating on magnesium Bo Li.et al,Materials Chemistry B,2016 (4):683-693 Interleukin-22 ameliorates liver fibrosis through miR-200a/beta-catenin Hu BL.et al,Scientific Reports,Nov 7;6:36436 Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine Yang C.et al,Cell Physiol Biochem ,/ Black Phosphorus Hydrogel Scaffolds Enhance Bone Regeneration via a Sustained Supply of Calcium-Free Phosphorus Huang K, et al,Acs Applied Materials & Interfaces,December 31

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10501879	CSB-E08082h	96T	$835	Add to Cart
BHE10501879	CSB-E08082h	5x96T	$4008	Add to Cart
BHE10501879	CSB-E08082h	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.