Human collagen, type I, alpha 1 (COL1A1) ELISA kit

Human collagen, type I, alpha 1 (COL1A1) ELISA kit

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Mfr.No.

CSB-E13445h

Alternative Names

collagen, type I, alpha 1, OI4, alpha 1 type I collagen, collagen alpha 1 chain type I, collagen of skin, tendon and bone, alpha-1 chain, pro-alpha-1 collagen type 1, collagen, type I, alpha 1, OI4, alpha 1 type I collagen, collagen alpha 1 chain type I, collagen of skin, tendon and bone, alpha-1 chain, pro-alpha-1 collagen type 1

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CASP-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-103
EDTA plasma (n=4)	97	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CASP-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-96
1:2	Average %	90
	Range %	84-102
1:4	Average %	95
	Range %	92-100
1:8	Average %	93
	Range %	83-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.226	2.238	2.232	2.136
1000	1.513	1.546	1.530	1.434
500	0.917	0.932	0.925	0.829
250	0.588	0.579	0.584	0.488
125	0.406	0.394	0.400	0.304
62.5	0.251	0.237	0.244	0.148
31.25	0.139	0.133	0.136	0.040
0	0.095	0.097	0.096

Species

Homo sapiens

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	P02452
Antigen	collagen, type I, alpha 1

Applications

ELISA

Research Area

COL1A1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501884	CSB-E13445h	96T	$790	Add to Cart
BHE10501884	CSB-E13445h	5x96T	$3792	Add to Cart
BHE10501884	CSB-E13445h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.