Human Collagen alpha-2(IV) chain (COL4A2) ELISA kit

Human Collagen alpha-2(IV) chain (COL4A2) ELISA kit

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Mfr.No.

CSB-EL005742HU

Sensitivity

11.75 pg/mL

Detection Range

47 pg/mL-3000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

collagen, type IV, alpha 2, RP11-90L1.2, DKFZp686I14213, FLJ22259, alpha 2 type IV collagen, canstatin, collagen, type IV, alpha 2, RP11-90L1.2, DKFZp686I14213, FLJ22259, alpha 2 type IV collagen, canstatin

Recovery

The recovery of bovine interferon-tau spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-96
EDTA plasma (n=4)	96	91-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine interferon-tau in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-92
1:2	Average %	98
	Range %	94-101
1:4	Average %	92
	Range %	87-98
1:8	Average %	95
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.587	2.558	2.573	2.485
200	2.140	2.003	2.072	1.984
100	1.644	1.621	1.633	1.545
50	1.109	1.027	1.068	0.980
25	0.698	0.687	0.693	0.605
12.5	0.399	0.387	0.393	0.305
6.25	0.242	0.231	0.237	0.149
0	0.090	0.086	0.088	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08572

Applications	ELISA
Research Area	COL4A2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501864	CSB-EL005742HU	96T	$695	Add to Cart
BHE10501864	CSB-EL005742HU	5x96T	$3336	Add to Cart
BHE10501864	CSB-EL005742HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.