Human Collagen alpha-2(I) chain (COL1A2) ELISA kit

Human Collagen alpha-2(I) chain (COL1A2) ELISA kit

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Mfr.No.

CSB-EL005728HU

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

collagen, type I, alpha 2, OI4, alpha 2 type I collagen, alpha 2(I)-collagen, collagen I, alpha-2 polypeptide, collagen of skin, tendon and bone, alpha-2 chain, type I procollagen, collagen, type I, alpha 2, OI4, alpha 2 type I collagen, alpha 2(I)-collagen, collagen I, alpha-2 polypeptide, collagen of skin, tendon and bone, alpha-2 chain, type I procollagen

Recovery

The recovery of mouse sTREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	100	91-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse sTREM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	86-99
1:2	Average %	98
	Range %	90-102
1:4	Average %	92
	Range %	85-96
1:8	Average %	98
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.657	2.542	2.600	2.470
500	2.038	2.089	2.064	1.934
250	1.363	1.414	1.389	1.259
125	0.756	0.788	0.772	0.642
62.5	0.463	0.454	0.459	0.329
31.3	0.321	0.313	0.317	0.187
15.6	0.256	0.266	0.261	0.131
0	0.132	0.128	0.130

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08123

Applications	ELISA
Research Area	COL1A2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501863	CSB-EL005728HU	96T	$595	Add to Cart
BHE10501863	CSB-EL005728HU	5x96T	$2856	Add to Cart
BHE10501863	CSB-EL005728HU	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.