Human Collagen alpha-1(XVII) chain (COL17A1) ELISA kit

Mfr.No.

CSB-EL005724HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

collagen, type XVII, alpha 1, RP11-16H23.2, BA16H23.2, BP180, BPAG2, FLJ60881, KIAA0204, LAD-1, alpha 1 type XVII collagen, bA16H23.2 (collagen, type XVII, alpha 1 (BP180)), bullous pemphigoid antigen 2 (180kD), collagen XVII, alph, collagen, type XVII, alpha 1, RP11-16H23.2, BA16H23.2, BP180, BPAG2, FLJ60881, KIAA0204, LAD-1, alpha 1 type XVII collagen, bA16H23.2 (collagen, type XVII, alpha 1 (BP180)), bullous pemphigoid antigen 2 (180kD), collagen XVII, alph

Recovery

The recovery of rat NTS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	100	95-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat NTS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	84
	Range %	80-88
1:4	Average %	95
	Range %	90-99
1:8	Average %	89
	Range %	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.541	2.379	2.460	2.253
500	1.839	1.721	1.780	1.573
250	1.152	1.205	1.179	0.972
125	0.785	0.816	0.801	0.594
62.5	0.503	0.482	0.493	0.286
31.2	0.348	0.328	0.338	0.131
15.6	0.294	0.303	0.299	0.092
0	0.203	0.210	0.207	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9UMD9

Applications	ELISA
Research Area	COL17A1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501862	CSB-EL005724HU	96T	$695	Add to Cart
BHE10501862	CSB-EL005724HU	5x96T	$3336	Add to Cart
BHE10501862	CSB-EL005724HU	10x96T	$6602.50	Add to Cart

Human Collagen alpha-1(XVII) chain (COL17A1) ELISA kit

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