Human Collagen alpha-1(XI) chain (COL11A1) ELISA kit

Mfr.No.

CSB-EL005716HU

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

collagen, type XI, alpha 1, CO11A1, COLL6, STL2, alpha 1 type XI collagen, collagen XI, alpha-1 polypeptide, collagen, type XI, alpha 1, CO11A1, COLL6, STL2, alpha 1 type XI collagen, collagen XI, alpha-1 polypeptide

Recovery

The recovery of mouse NTS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	97	94-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse NTS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-89
1:2	Average %	98
	Range %	94-105
1:4	Average %	90
	Range %	85-95
1:8	Average %	107
	Range %	103-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.890	2.768	2.829	2.637
200	2.346	2.204	2.275	2.083
100	1.626	1.702	1.664	1.472
50	1.047	1.100	1.074	0.882
25	0.733	0.741	0.737	0.545
12.5	0.517	0.502	0.510	0.318
6.25	0.371	0.382	0.377	0.185
0	0.191	0.193	0.192	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P12107

Applications	ELISA
Research Area	COL11A1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501855	CSB-EL005716HU	96T	$695	Add to Cart
BHE10501855	CSB-EL005716HU	5x96T	$3336	Add to Cart
BHE10501855	CSB-EL005716HU	10x96T	$6602.50	Add to Cart

Human Collagen alpha-1(XI) chain (COL11A1) ELISA kit

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