Human Cold-inducible RNA-binding Protein

Mfr.No.

E5066Hu

Sensitivity

4.02ng/L

Detection Range

7-1500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

A18 hnRNP;CIRBP;Cold-inducible RNA-binding protein;Glycine-rich RNA-binding protein CIRP

Molecular Weight

18648

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5066Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	112.6	6.16	5.5
Intra-Assay	2	18	525.3	16.06	3.1
Intra-Assay	3	18	329.3	17.37	5.3

Background

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.

Protocol

These standard curves of E5066Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.535	2.536	2.489
	2.538
400ng/L	1.385	1.424	1.376
	1.462
200ng/L	0.801	0.782	0.734
	0.762
100ng/L	0.518	0.466	0.419
	0.415
50ng/L	0.266	0.239	0.192
	0.212
0ng/L	0.034	0.047	0
	0.061

Gene ID	1153
UniProt ID	Q14011

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106429	E5066Hu	48T	$320		Add to Cart
BHE12106429	E5066Hu	96T	$458		Add to Cart

Human Cold-inducible RNA-binding Protein - CIRBP ELISA Kit

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