Matrices listed below were spiked with certain level of COL17 and the recovery rates were calculated by comparing the measured value to the expected amount of COL17 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
96
EDTA plasma(n=5)
92-102
97
heparin plasma(n=5)
86-102
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
COL17 ELISA Kit allows for the in vitro quantitative determination of COL17 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human COL17(Collagen Type XVII) ELISA Kit