Matrices listed below were spiked with certain level of COL15 and the recovery rates were calculated by comparing the measured value to the expected amount of COL15 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-101
92
EDTA plasma(n=5)
87-102
97
heparin plasma(n=5)
89-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
COL15 ELISA Kit allows for the in vitro quantitative determination of COL15 concentrations in serum, plasma, tissue homogenates and other biological fluids.