Human Cohesin Subunit Sa-2 - STAG2 ELISA Kit

Mfr.No.

E2958Hu

Sensitivity

5.08ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Cohesin subunit SA-2;SCC3 homolog 2;STAG 2;STAG2;Stromal antigen 2

Molecular Weight

141326

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2958Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	876.2	34.08	3.9
Intra-Assay	2	18	228.6	13.9	6.1
Intra-Assay	3	18	483.9	27.74	5.7

Background

Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

Protocol

These standard curves of E2958Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.229	2.17	2.106
	2.111
600ng/L	1.289	1.295	1.23
	1.3
300ng/L	0.833	0.831	0.767
	0.83
150ng/L	0.407	0.403	0.339
	0.4
75ng/L	0.297	0.277	0.212
	0.257
0ng/L	0.053	0.064	0
	0.076

Gene ID	10735
UniProt ID	Q8N3U4

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104495	E2958Hu	48T	$320		Add to Cart
BHE12104495	E2958Hu	96T	$458		Add to Cart

Human Cohesin Subunit Sa-2 - STAG2 ELISA Kit

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