Human Coenzyme Q10 (CoQ10) ELISA Kit

Mfr.No.

CSB-E14081h

Alternative Names

Coenzyme Q10 (CoQ10)

Reactivity

Human

Detection Range

3.12 ng/mL-50 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human EPHB2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-95
EDTA plasma (n=4)	96	92-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EPHB2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-96
1:2	Average %	96
	Range %	90-101
1:4	Average %	97
	Range %	90-104
1:8	Average %	101
	Range %	96-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.607	2.688	2.648	2.462
20	1.935	1.985	1.960	1.774
10	1.125	1.212	1.169	0.983
5	0.683	0.698	0.691	0.505
2.5	0.442	0.447	0.445	0.259
1.25	0.309	0.323	0.316	0.130
0.625	0.230	0.246	0.238	0.052
0	0.182	0.190	0.186	?

Species

Homo sapiens

Sensitivity

1.56 ng/mL

Limit Of Detection

450 nm

Antigen	Coenzyme Q10 (CoQ10)

Applications

ELISA

Research Area

CoQ10

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The Effect of Coenzyme Q10 Administration on DNA Damage in Diabetic Polyneuropathy Sandra Carrillo-Ibarra.et al,Journal of Global Diabetes & Clinical Metabolism,May 8 The effect of Ubiquinoneadministration on oxidative DNAdamage and repair in plasma levels innon-proliferative diabetic retinopathy Sonia Sifuentes-Franco1.et al,Diabetes Management,/

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SKU	Supplier No.	Size	Your price
BHE10501841	CSB-E14081h	96T	$790	Add to Cart
BHE10501841	CSB-E14081h	5x96T	$3792	Add to Cart
BHE10501841	CSB-E14081h	10x96T	$7505	Add to Cart

Human Coenzyme Q10 (CoQ10) ELISA Kit

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