Human Coagulation Factor XIII A1 Polypeptide(F13A1) ELISA kit

Human Coagulation Factor XIII A1 Polypeptide(F13A1) ELISA kit

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Mfr.No.

CSB-E14291h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

coagulation factor XIII, A1 polypeptide, RP11-232H4.1, F13A, FSF, A subunit, TGase, bA525O21.1 (coagulation factor XIII, A1 polypeptide), coagulation factor XIII A1 subunit, coagulation factor XIII, A polypeptide, factor XIIIa, fibrin stabilizin

Recovery

The recovery of chicken EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	102-106
EDTA plasma (n=4)	93	90-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	100-104
1:2	Average %	95
	Range %	92-98
1:4	Average %	88
	Range %	85-91
1:8	Average %	99
	Range %	96-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.337	2.466	2.402	2.289
4000	1.893	1.875	1.884	1.771
2000	1.452	1.455	1.454	1.341
1000	1.064	1.099	1.082	0.969
500	0.643	0.632	0.638	0.525
250	0.443	0.478	0.461	0.348
125	0.272	0.295	0.284	0.171
0	0.114	0.112	0.113

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P00488

Applications	ELISA
Research Area	F13A1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501838	CSB-E14291h	96T	$790	Add to Cart
BHE10501838	CSB-E14291h	5x96T	$3792	Add to Cart
BHE10501838	CSB-E14291h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.