Human coagulation factor VIII (FVIII) ELISA Kit

Human coagulation factor VIII (FVIII) ELISA Kit

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Mfr.No.

CSB-E13861h

Sensitivity

3.12 ng/mL

Detection Range

12.5 ng/mL-800 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

coagulation factor VIII, procoagulant component, RP11-115M6.7, AHF, DXS1253E, F8B, F8C, FVIII, HEMA, coagulation factor VIII, coagulation factor VIIIc, factor VIII F8B, coagulation factor 8 (F8), coagulation factor VIII (FVIII)

Recovery

The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	101	99-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-105
1:2	Average %	93
	Range %	90-96
1:4	Average %	102
	Range %	98-106
1:8	Average %	87
	Range %	83-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
10000	2.647	2.687	2.667
5000	1.839	1.899	1.869
2500	1.057	1.067	1.062
1250	0.599	0.584	0.592
500	0.241	0.231	0.236
250	0.112	0.114	0.113

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P00451

Applications	ELISA
Research Area	F8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501835	CSB-E13861h	96T	$790	Add to Cart
BHE10501835	CSB-E13861h	5x96T	$3792	Add to Cart
BHE10501835	CSB-E13861h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.