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Home / ELISA Kit

Human coagulation factor IX,FIX ELISA Kit

SKU : BHE10501832

This Human TNFSF13 ELISA Kit was designed for the quantitative measurement of Human TNFSF13 protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E08443h

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of human APRIL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		104		100-108
EDTA plasma (n=4)		97		92-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APRIL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		90-98
1:2		Average %		92
		Range %		88-97
1:4		Average %		86
		Range %		82-90
1:8		Average %		98
		Range %		94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.285	2.352	2.319	2.179
25	1.605	1.565	1.585	1.445
12.5	1.053	1.104	1.079	0.939
6.25	0.656	0.679	0.668	0.528
3.12	0.411	0.396	0.404	0.264
1.56	0.298	0.318	0.308	0.168
0.78	0.212	0.209	0.211	0.071
0	0.143	0.137	0.140

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	P00740

Applications	ELISA
Research Area	F9

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10501832	CSB-E08443h	96T	$595		Add to Cart
BHE10501832	CSB-E08443h	5x96T	$2856		Add to Cart
BHE10501832	CSB-E08443h	10x96T	$5652.50		Add to Cart

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