Human Coagulation Factor 5

Mfr.No.

E4114hu

Sensitivity

0.53ng/mL

Detection Range

1-400ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Activated protein C cofactor;Coagulation factor V;Coagulation factor V heavy chain;Coagulation factor V light chain;F 5;F5;Proaccelerin, labile factor

Molecular Weight

251703

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4114hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	15	0.67	4.5
Intra-Assay	2	18	79.7	3.39	4.2
Intra-Assay	3	18	48.9	2.93	6

Background

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

Protocol

These standard curves of E4114hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/mL	2.23	2.165	2.122
	2.1
120ng/mL	1.772	1.847	1.805
	1.923
60ng/mL	1.132	1.155	1.112
	1.178
30ng/mL	0.678	0.673	0.63
	0.668
15ng/mL	0.421	0.396	0.353
	0.371
0ng/mL	0.047	0.043	0
	0.039

Gene ID	2153
UniProt ID	P12259

Research Area	Blood Coagulation

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105519	E4114hu	48T	$320		Add to Cart
BHE12105519	E4114hu	96T	$458		Add to Cart

Human Coagulation Factor 5 - F5 ELISA Kit

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