Matrices listed below were spiked with certain level of CML and the recovery rates were calculated by comparing the measured value to the expected amount of CML in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
91
EDTA plasma(n=5)
86-105
97
heparin plasma(n=5)
93-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CML and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CML ELISA Kit allows for the in vitro quantitative determination of CML concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CML(Carboxymethyl Lysine) ELISA Kit