Human Cluster of differentiation 8,CD8 ELISA Kit

Mfr.No.

CSB-E12707h

Alternative Names

CD8a molecule, CD8, Leu2, MAL, p32, CD8 antigen alpha polypeptide, CD8 antigen, alpha polypeptide (p32), Leu2 T-lymphocyte antigen, OKT8 T-cell antigen, OTTHUMP00000203721, T cell co-receptor, T-cell antigen Leu2, T-cell

Reactivity

Human

Detection Range

62.5 U/mL-4000 U/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	82-102
EDTA plasma (n=4)	92	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-103
1:2	Average %	89
	Range %	85-96
1:4	Average %	93
	Range %	85-99
1:8	Average %	95
	Range %	87-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.244	2.237	2.241	2.103
30	1.985	1.992	1.989	1.851
15	1.608	1.613	1.611	1.473
7.5	1.137	1.155	1.146	1.008
3.75	0.702	0.714	0.708	0.570
1.8	0.506	0.512	0.509	0.371
0.9	0.351	0.359	0.355	0.217
0	0.137	0.139	0.138	?

Species

Homo sapiens

Sensitivity

15.6 U/mL

Limit Of Detection

450 nm

UniProt ID	P01732
Antigen	CD8a molecule

Applications

ELISA

Research Area

CD8A

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501824	CSB-E12707h	96T	$595	Add to Cart
BHE10501824	CSB-E12707h	5x96T	$2856	Add to Cart
BHE10501824	CSB-E12707h	10x96T	$5652.50	Add to Cart

Human Cluster of differentiation 8,CD8 ELISA Kit

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