Human cluster of differentiation 73(CD73) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14099h

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

cluster of differentiation 73(CD73)

Recovery

The recovery of sheep PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-104
EDTA plasma (n=4)	95	88-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	94
	Range %	84-101
1:4	Average %	93
	Range %	85-103
1:8	Average %	90
	Range %	87-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














?IU/ml	OD1	OD2	Average
2000	0.154	0.149	0.152
375	0.338	0.351	0.345
62.5	0.542	0.572	0.557
17.5	0.983	0.959	0.971
5	1.515	1.529	1.522
0	2.164	2.077	2.121

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P21589

Applications	ELISA
Research Area	CD73

Storage	Store at 2-8°C. Please refer to protocol.

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