Human cluster of differentiation 63,CD63 ELISA Kit

Human cluster of differentiation 63,CD63 ELISA Kit

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Mfr.No.

CSB-E14107h

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

CD63 molecule, LAMP-3, ME491, MLA1, OMA81H, TSPAN30, CD63 antigen, CD63 antigen (melanoma 1 antigen), granulophysin, lysosome-associated membrane glycoprotein 3, melanoma 1 antigen, melanoma-associated antigen ME491, me

Recovery

The recovery of mouse oxyntomodulin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	91	86-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse oxyntomodulin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	102
	Range %	92-108
1:10	Average %	89
	Range %	85-96
1:20	Average %	97
	Range %	91-105
1:40	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
20	2.415	2.514	2.465	2.282
10	1.687	1.786	1.737	1.554
5	1.067	0.999	1.033	0.850
2.5	0.657	0.648	0.653	0.470
1.25	0.436	0.427	0.432	0.249
0.625	0.312	0.303	0.308	0.125
0.312	0.244	0.249	0.247	0.064
0	0.182	0.184	0.183	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P08962
Tissue Specificity	Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

Applications	ELISA
Research Area	CD63

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501819	CSB-E14107h	96T	$695	Add to Cart
BHE10501819	CSB-E14107h	5x96T	$3336	Add to Cart
BHE10501819	CSB-E14107h	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.