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Home / ELISA Kit

Human Cluster of differentiation 30 (CD30) ELISA Kit

SKU : BHE13702429

This protein is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

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Mfr.No.	KTE60211
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Cluster of differentiation 30 (CD30) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF8.
Alternative Names	TNFRSF8; CD30; D1S166E; Ki-1; CD30 antigen; CD30L receptor; Ki-1 antigen; cytokine receptor CD30; lymphocyte activation antigen CD30
Detection Method	Colorimetric
Kit Components	Human Cluster of differentiation 30 microplate Human Cluster of differentiation 30 standard Human Cluster of differentiation 30 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Cluster of differentiation 30 (CD30) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFRSF8. No significant cross-reactivity or interference between Human TNFRSF8 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	This protein is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Notes	This Human Cluster of differentiation 30 (CD30) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF8 in samples. An antibody specific for TNFRSF8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	943

Applications	ELISA
Applications Notes	This Human Cluster of differentiation 30 (CD30) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF8 in samples. An antibody specific for TNFRSF8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702429	KTE60211	48 T	$339		Add to Cart
BHE13702429	KTE60211	96 T	$589		Add to Cart
BHE13702429	KTE60211	96 Tx5	$2629		Add to Cart
BHE13702429	KTE60211	96 Tx50	$24569		Add to Cart

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