Human Cluster of Differentiation 3

Mfr.No.

E0221Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Cluster Of Differentiation 3 (also known as CD3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

E1A-like inhibitor of differentiation 3;EID 3;EID-1-like inhibitor of differentiation 3;EID3;EID-3;EP300-interacting inhibitor of differentiation 3;Non-SMC element 4 homolog B;Non-structural maintenance of chromosomes element 4 homolog B;NS4EB;

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

38168

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.01ng/ml

Background

Tissue-specific component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components. Acts as a repressor of nuclear receptor-dependent transcription possibly by interfering with CREBBP-dependent coactivation. May function as a coinhibitor of other CREBBP/EP300-dependent transcription factors.

Gene ID	493861
UniProt ID	Q8N140
Antigen	EID3

Research Area

Others

Protocol

These standard curves of E0221Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.218	2.272	2.23
	2.325
6ng/ml	1.426	1.331	1.289
	1.236
3ng/ml	0.585	0.64	0.598
	0.695
1.5ng/ml	0.336	0.359	0.317
	0.381
0.75ng/ml	0.181	0.166	0.124
	0.15
0ng/ml	0.039	0.042	0
	0.045

Precision

We measured random samples of E0221Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.1	0.09	4.4
Intra-Assay	2	18	5.6	0.33	5.9
Intra-Assay	3	18	6.4	0.18	2.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101938	E0221Hu	96T	$458		Add to Cart
BHE12101938	E0221Hu	48T	$320		Add to Cart

Human Cluster of Differentiation 3 - CD3 ELISA Kit

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