Matrices listed below were spiked with certain level of CLIC1 and the recovery rates were calculated by comparing the measured value to the expected amount of CLIC1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-105
99
EDTA plasma(n=5)
86-102
95
heparin plasma(n=5)
90-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CLIC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-102%
93-104%
85-102%
EDTA plasma(n=5)
82-98%
85-101%
82-99%
heparin plasma(n=5)
80-98%
88-98%
80-98%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
O00299
Applications
ELISA
Applications Notes
CLIC1(Chloride intracellular channel protein 1) ELISA Kit allows for the in vitro quantitative determination of CLIC1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CLIC1(Chloride intracellular channel protein 1) ELISA Kit