Matrices listed below were spiked with certain level of Cleaved CASP9 and the recovery rates were calculated by comparing the measured value to the expected amount of Cleaved CASP9 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
97
EDTA plasma(n=5)
88-101
95
heparin plasma(n=5)
87-97
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cleaved CASP9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Cleaved CASP9 ELISA Kit allows for the in vitro quantitative determination of Cleaved CASP9 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human Cleaved CASP9(Cleaved Caspase-9) ELISA Kit