Matrices listed below were spiked with certain level of Cleaved CASP8 and the recovery rates were calculated by comparing the measured value to the expected amount of Cleaved CASP8 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
95
EDTA plasma(n=5)
88-103
99
heparin plasma(n=5)
86-105
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cleaved CASP8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Cleaved CASP8 ELISA Kit allows for the in vitro quantitative determination of Cleaved CASP8 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human Cleaved CASP8(Cleaved Caspase-8) ELISA Kit