Human Claudin-5(CLDN5) ELISA kit

Human Claudin-5(CLDN5) ELISA kit

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Mfr.No.

CSB-EL005507HU

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

claudin 5, AWAL, BEC1, CPETRL1, TMVCF, OTTHUMP00000197372, OTTHUMP00000197819, androgen withdrawal and apoptosis induced protein RVP1-like, transmembrane protein deleted in velocardiofacial syndrome

Recovery

The recovery of human NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	96	92-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-95
1:2	Average %	103
	Range %	99-106
1:4	Average %	93
	Range %	87-96
1:8	Average %	97
	Range %	93-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.160	2.145	2.153	2.013
20	1.676	1.740	1.708	1.568
10	1.208	1.188	1.198	1.058
5	0.862	0.826	0.844	0.704
2.5	0.602	0.616	0.609	0.469
1.25	0.442	0.434	0.438	0.298
0.625	0.296	0.278	0.287	0.147
0	0.142	0.138	0.140	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O00501

Applications	ELISA
Research Area	CLDN5

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501803	CSB-EL005507HU	96T	$695	Add to Cart
BHE10501803	CSB-EL005507HU	5x96T	$3336	Add to Cart
BHE10501803	CSB-EL005507HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.