Human Claudin 4 (CLDN4)ELISA Kit

Human Claudin 4 (CLDN4)ELISA Kit

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Mfr.No.

CSB-E17961h

Alternative Names

claudin 4, CPE-R, CPER, CPETR, CPETR1, WBSCR8, hCPE-R, Clostridium perfringens enterotoxin receptor 1, OTTHUMP00000160507, OTTHUMP00000211778, Williams-Beuren syndrome chromosomal region 8 protein

Reactivity

Human

Detection Range

6.25 pg/mL-400 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rat FASL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	87	81-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat FASL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	88
	Range %	82-94
1:4	Average %	101
	Range %	95-104
1:8	Average %	92
	Range %	89-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.212	2.403	2.308	2.166
1000	1.647	1.554	1.601	1.459
500	1.005	0.988	0.997	0.855
250	0.592	0.587	0.590	0.448
125	0.413	0.402	0.408	0.266
62.5	0.272	0.264	0.268	0.126
31.25	0.188	0.192	0.190	0.048
0	0.143	0.141	0.142

Species

Homo sapiens

Sensitivity

1.56 pg/mL

Limit Of Detection

450 nm

UniProt ID	O14493
Antigen	Claudin 4

Applications

ELISA

Research Area

CLDN4

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10501802	CSB-E17961h	5x96T	$3792	Add to Cart
BHE10501802	CSB-E17961h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.