Human Clara cell protein,CC16 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08680h

Reactivity

Human

Detection Range

0.625 ng/ml - 40 ng/ml

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse FGL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	107	104-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-99
1:200	Average %	84
	Range %	81-89
1:400	Average %	83
	Range %	90-96
1:800	Average %	87
	Range %	84-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.156 ng/ml

Limit Of Detection

450 nm

UniProt ID	P11684
Antigen	Clara cell protein,CC16

Applications

ELISA

Research Area

CC16

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Global DNA hypomethylation has no impact on lung function or serum inflammatory and fibrosis cytokines in asbestos-exposed population Yu M.et al,Int Arch Occup Environ Health. ,Jan 19

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