Human ckmm ELISA Kit PicoKine®

Mfr.No.

EK1751

Sensitivity

<0.05 mU/ml

Detection Range

0.15mU/ml - 10mU/ml

Reactivity

Human

Sample Volume

Concentration (mU/ml)	0	0.15	0.31	0.62	1.25	2.5	5	10
O.D.	0.021	0.040	0.068	0.109	0.183	0.384	0.872	1.829

Description

Human ckmm ELISA Kit PicoKine® (96 Tests). Quantitate Human CKM in cell culture supeatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 0.05mU/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Creatine kinase M-type; Creatine kinase M chain; Creatine kinase M chain; CPK-M; CKM; CKMM

Immunogen

natural protein

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human ckmm

Subcellular Localization

Cytoplasm

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (mU/ml)	233	1369	3636	242	1421	4520
Standard deviation	9.77	88.99	147.5	13.31	96.6	329.96
CV (%)	4.2%	6.5%	4.8%	5.5%	6.8%	7.3%

Protein Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle,

Background

Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Reproducibility

Lots	Lot 1 (mU/ml)	Lot 2 (mU/ml)	Lot 3 (mU/ml)	Lot 4 (mU/ml)	Mean (mU/ml)	Standard Deviation	CV (%)
Sample 1	233	233	252	234	238	8.09	3.3%
Sample 2	1369	1559	1602	1564	1523	90.73	5.9%
Sample 3	3636	3701	3777	4293	3851	259.59	6.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1158
Gene Name	CKM
UniProt ID	P06732

Applications	ELISA
Research Area	Energy Metabolism,Energy Transfer Pathways,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001385	EK1751	96 wells/kit	$499		Add to Cart

Human ckmm ELISA Kit PicoKine®

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