Matrices listed below were spiked with certain level of CK-BB and the recovery rates were calculated by comparing the measured value to the expected amount of CK-BB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
97
EDTA plasma(n=5)
87-103
95
heparin plasma(n=5)
86-95
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK-BB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
90-104%
96-104%
EDTA plasma(n=5)
83-101%
87-99%
87-92%
heparin plasma(n=5)
80-99%
80-98%
87-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
CK-BB ELISA Kit allows for the in vitro quantitative determination of CK-BB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CK-BB (Creatine Kinase BB) ELISA Kit