Human CK 18-M30 (Cytokeratin 18-M30) ELISA kit

Mfr.No.

ELK0871

Sensitivity

7.1 uIU/mL

Detection Range

15.63-1000 uIU/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

K18-M30; CK-18-M30; CYK18-M30

Recovery

Matrices listed below were spiked with certain level of recombinant CK 18-M30 and the recovery rates were calculated by comparing the measured value to the expected amount of CK 18-M30 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-95%	90%
EDTA plasma(n=5)	88-103%	95%
Heparin plasma(n=5)	83-95%	89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK 18-M30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	88-97%	91-103%	86-92%	89-97%
EDTA plasma(n=5)	88-104%	87-96%	85-93%	82-90%
Heparin plasma(n=5)	95-102%	89-96%	87-98%	85-93%

Typical Data

Concentration (?IU/mL)	OD	Corrected OD
1000.00	1.972	1.883
500.00	1.507	1.418
250.00	1.107	1.018
125.00	0.671	0.582
62.50	0.479	0.390
31.25	0.312	0.223
15.63	0.196	0.107
0.00	0.089	0.000

Sample Type

Cell lysates, Serum, Cell culture supeatants, Tissue homogenates, Plasma, Other biological fluids

Limit Of Detection

1000 ?IU/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK 18-M30. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK 18-M30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK 18-M30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK 18-M30 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Cancer of the liver; Viral hepatitis

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208403	ELK0871	48T	$320		Add to Cart
BHE15208403	ELK0871	96T	$458		Add to Cart

Human CK 18-M30 (Cytokeratin 18-M30) ELISA kit

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