Human Chromogranin,CgA ELISA Kit

Human Chromogranin,CgA ELISA Kit

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Mfr.No.

CSB-E09153h

Alternative Names

chromogranin A (parathyroid secretory protein 1), CGA, betagranin (N-terminal fragment of chromogranin A), chromogranin A, parathyroid secretory protein 1

Reactivity

Human

Detection Range

62.5 ng/mL-4000 ng/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Urine, Cerebrospinal fluid

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse FAM132B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	89	84-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FAM132B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	93
	Range %	87-99
1:4	Average %	102
	Range %	98-107
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.388	2.533	2.461	2.297
600	1.560	1.588	1.574	1.410
300	0.978	0.901	0.940	0.776
150	0.622	0.602	0.612	0.448
75	0.363	0.355	0.359	0.195
37.5	0.274	0.295	0.285	0.121
18.75	0.189	0.202	0.196	0.032
0	0.159	0.168	0.164

Species

Homo sapiens

Sensitivity

15.6 ng/mL

Limit Of Detection

450 nm

UniProt ID	P10645
Antigen	chromogranin A (parathyroid secretory protein 1)

Applications

ELISA

Research Area

CHGA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501788	CSB-E09153h	96T	$790	Add to Cart
BHE10501788	CSB-E09153h	5x96T	$3792	Add to Cart
BHE10501788	CSB-E09153h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.