Human Chondroitin sulfate proteoglycan 4(CSPG4) ELISA kit

Human Chondroitin sulfate proteoglycan 4(CSPG4) ELISA kit

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Mfr.No.

CSB-EL006076HU

Alternative Names

chondroitin sulfate proteoglycan 4, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2, chondroitin sulfate proteoglycan 4 (melanoma-associated), melanoma-associated chondroitin sulfate proteoglycan

Reactivity

Human

Detection Range

25 pg/mL-1600 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse Acetyl-CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	92-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Acetyl-CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	91
	Range %	87-93
1:40	Average %	103
	Range %	100-105
1:80	Average %	93
	Range %	87-96
1:160	Average %	98
	Range %	93-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.192	2.114	2.153	1.996
25	1.632	1.647	1.640	1.483
12.5	1.124	1.110	1.117	0.960
6.25	0.713	0.688	0.701	0.544
3.12	0.404	0.421	0.413	0.256
1.56	0.291	0.278	0.285	0.128
0.78	0.220	0.212	0.216	0.059
0	0.158	0.156	0.157	?

Species

Homo sapiens

Sensitivity

6.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q6UVK1
Antigen	chondroitin sulfate proteoglycan 4

Applications

ELISA

Research Area

CSPG4

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501780	CSB-EL006076HU	96T	$695	Add to Cart
BHE10501780	CSB-EL006076HU	5x96T	$3336	Add to Cart
BHE10501780	CSB-EL006076HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.