Human Cholinesterase - BCHE ELISA Kit

Mfr.No.

E5042Hu

Sensitivity

1.07U/mL

Detection Range

2-600U/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Acylcholine acylhydrolase;BCHE;Butyrylcholine esterase;Choline esterase II;Cholinesterase;Pseudocholinesterase

Molecular Weight

68418

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5042Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	43.6	2.28	5.2
Intra-Assay	2	18	136.3	3.87	2.8
Intra-Assay	3	18	80.2	4.22	5.3

Background

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

Protocol

These standard curves of E5042Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320U/mL	2.292	2.256	2.202
	2.22
160U/mL	1.455	1.422	1.368
	1.388
80U/mL	0.95	0.91	0.856
	0.87
40U/mL	0.447	0.432	0.378
	0.416
20U/mL	0.217	0.245	0.191
	0.272
0U/mL	0.055	0.054	0
	0.052

Gene ID	590
UniProt ID	P06276

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106405	E5042Hu	48T	$320		Add to Cart
BHE12106405	E5042Hu	96T	$458		Add to Cart

Human Cholinesterase - BCHE ELISA Kit

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