Human Cholinesterase - BCHE ELISA Kit

Mfr.No.

E5042Hu

Alternative Names

Acylcholine acylhydrolase;BCHE;Butyrylcholine esterase;Choline esterase II;Cholinesterase;Pseudocholinesterase

Reactivity

Human

Detection Range

2-600U/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

68418

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.07U/mL

Background

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

Gene ID	590
UniProt ID	P06276
Antigen	BCHE

Research Area

Neuroscience

Protocol

These standard curves of E5042Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320U/mL	2.292	2.256	2.202
	2.22
160U/mL	1.455	1.422	1.368
	1.388
80U/mL	0.95	0.91	0.856
	0.87
40U/mL	0.447	0.432	0.378
	0.416
20U/mL	0.217	0.245	0.191
	0.272
0U/mL	0.055	0.054	0
	0.052

Precision

We measured random samples of E5042Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	43.6	2.28	5.2
Intra-Assay	2	18	136.3	3.87	2.8
Intra-Assay	3	18	80.2	4.22	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106405	E5042Hu	48T	$320		Add to Cart
BHE12106405	E5042Hu	96T	$458		Add to Cart

Human Cholinesterase - BCHE ELISA Kit

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