Human Cholecystokinin - CCK ELISA Kit

Mfr.No.

E1357Hu

Sensitivity

1.32ng/L

Detection Range

2-100ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Cholecystokinin (also known as CCK) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

(1-49-CCK58;CCK;CCK12;CCK18;CCK25;CCK33;CCK39;CCK5;CCK58;CCK7;CCK8;Cholecystokinin;Cholecystokinin-12;Cholecystokinin-18;Cholecystokinin-25;Cholecystokinin-33;Cholecystokinin-39;Cholecystokinin-5;Cholecystokinin-58;Cholecystokinin-58 desnonopeptide;Cholecystokinin-7;Cholecystokinin-8;

Molecular Weight

12669

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1357Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	30.4	1.19	3.9
Intra-Assay	2	18	14.1	1.09	7.7
Intra-Assay	3	18	15.9	0.98	6.1

Background

This Peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.

Protocol

These standard curves of E1357Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/L	2.283	2.176	2.115
	2.069
32ng/L	1.347	1.317	1.256
	1.287
16ng/L	0.861	0.891	0.831
	0.921
8ng/L	0.464	0.436	0.375
	0.407
4ng/L	0.3	0.274	0.213
	0.248
0ng/L	0.051	0.061	0
	0.07

Gene ID	885
UniProt ID	P06307

Research Area	Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102995	E1357Hu	48T	$320		Add to Cart
BHE12102995	E1357Hu	96T	$458		Add to Cart

Human Cholecystokinin - CCK ELISA Kit

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