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Home / ELISA Kit

Human Chitinase-3-like protein 2(CHI3L2) ELISA kit

SKU : BHE10501764

This Human ORM2 ELISA Kit was designed for the quantitative measurement of Human ORM2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-EL005347HU

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of human ORM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		90		82-95
EDTA plasma (n=4)		94		90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ORM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		98
		Range %		90-102
1:200		Average %		89
		Range %		83-94
1:400		Average %		101
		Range %		93-105
1:800		Average %		105
		Range %		97-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
5000	0.131	0.135	0.133
2500	0.389	0.401	0.395
1250	0.750	0.732	0.741
625	1.042	1.020	1.031
312	1.441	1.454	1.448
156	1.650	1.666	1.658
78	1.806	1.767	1.787
0	2.001	2.078	2.040

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	Q15782

Applications	ELISA
Research Area	CHI3L2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10501764	CSB-EL005347HU	96T	$695		Add to Cart
BHE10501764	CSB-EL005347HU	5x96T	$3336		Add to Cart
BHE10501764	CSB-EL005347HU	10x96T	$6602.50		Add to Cart

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