Human chemerin ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E10398h

Sensitivity

7.8 ng/mL

Detection Range

31.2 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

retinoic acid receptor responder (tazarotene induced) 2, HP10433, TIG2, chemerin

Recovery

The recovery of human FGF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	91	86-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	89
	Range %	85-93
1:4	Average %	92
	Range %	88-96
1:8	Average %	106
	Range %	100-113

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q99969
Tissue Specificity	Expressed at the highest levels in placenta, liver, and white adipose tissue (WAT), and to a lesser extent in many other tissues such as lung, brown adipose tissue, heart, ovary, kidney, skeletal muscle and pancreas. Within WAT, expression is enriched in

Applications	ELISA
Research Area	RARRES2

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The association between polycystic ovary syndrome, obesity, and the serum concentration of adipokines Behboudi-Gandevani S.et al,J Endocrinol Invest. ,Mar 22. Evaluation of serum Chemerin level in non-diabetic, Hemodialized patients Saad M. Hussain Al-Obaidi, et al,Tikrit Joual of Pharmaceutical Sciences,/

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SKU	Supplier No.	Size	Your price	Quantity
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