Human chemerin ELISA Kit

Human chemerin ELISA Kit

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Mfr.No.

CSB-E10398h

Alternative Names

retinoic acid receptor responder (tazarotene induced) 2, HP10433, TIG2, chemerin

Reactivity

Human

Detection Range

31.2 pg/mL-2000 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human FGF5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	91	86-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	89
	Range %	85-93
1:4	Average %	92
	Range %	88-96
1:8	Average %	106
	Range %	100-113

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.314	2.227	2.271	2.126
500	1.816	1.876	1.846	1.701
250	1.334	1.402	1.368	1.223
125	0.801	0.840	0.821	0.676
62.5	0.544	0.518	0.531	0.386
31.2	0.372	0.359	0.366	0.221
15.6	0.271	0.282	0.277	0.132
0	0.142	0.147	0.145

Species

Homo sapiens

Sensitivity

7.8 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q99969
Antigen	retinoic acid receptor responder (tazarotene induced) 2

Applications

ELISA

Research Area

RARRES2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The association between polycystic ovary syndrome, obesity, and the serum concentration of adipokines Behboudi-Gandevani S.et al,J Endocrinol Invest. ,Mar 22. Evaluation of serum Chemerin level in non-diabetic, Hemodialized patients Saad M. Hussain Al-Obaidi, et al,Tikrit Journal of Pharmaceutical Sciences,/

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SKU	Supplier No.	Size	Your price	Quantity
BHE10501760	CSB-E10398h	96T	$790		Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.