Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of CERS6 and the recovery rates were calculated by comparing the measured value to the expected amount of CERS6 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
93
EDTA plasma(n=5)
87-105
98
heparin plasma(n=5)
90-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CERS6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-99%
86-103%
88-102%
EDTA plasma(n=5)
87-98%
88-98%
86-97%
heparin plasma(n=5)
92-100%
89-98%
85-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
CERS6 ELISA Kit allows for the in vitro quantitative determination of CERS6 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Q6ZMG9
Reviews of Human CERS6 (Ceramide synthase 6) ELISA Kit