Human Cerebral Dopamine Neurotrophic Factor

Mfr.No.

E2889Hu

Sensitivity

0.052ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Cerebral Dopamine Neurotrophic Factor (also known as CDNF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ARMETL1;ARMET-like protein 1;CDNF;Cerebral dopamine neurotrophic factor;Conserved dopamine neurotrophic factor

Molecular Weight

20964

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2889Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.31	0.42	4.46
Intra-Assay	2	18	15.14	0.49	3.23
Intra-Assay	3	18	4.65	0.29	6.23

Background

Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra (By similarity).

Protocol

These standard curves of E2889Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	2.311	2.246	2.202
	2.18
12ng/ml	1.251	1.306	1.263
	1.361
6ng/ml	0.838	0.786	0.742
	0.734
3ng/ml	0.48	0.493	0.449
	0.506
1.5ng/ml	0.198	0.213	0.17
	0.229
0ng/ml	0.041	0.044	0
	0.046

Gene ID	441549
UniProt ID	Q49AH0

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104426	E2889Hu	48T	$320		Add to Cart
BHE12104426	E2889Hu	96T	$458		Add to Cart

Human Cerebral Dopamine Neurotrophic Factor - CDNF ELISA Kit

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