Human Centromere Protein M - CENPM ELISA Kit

Mfr.No.

E5031Hu

Sensitivity

0.17ng/mL

Detection Range

0.3-90ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CENPM;CENP-M;Centromere protein M;Interphase centromere complex protein 39;Proliferation-associated nuclear element protein 1

Molecular Weight

19737

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5031Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	24.2	1.18	4.9
Intra-Assay	2	18	36.3	0.85	2.3
Intra-Assay	3	18	9.7	0.48	4.9

Background

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres.

Protocol

These standard curves of E5031Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.048	2.063	2.028
	2.078
24ng/mL	0.984	1.007	0.971
	1.03
12ng/mL	0.385	0.444	0.409
	0.503
6ng/mL	0.317	0.298	0.262
	0.278
3ng/mL	0.122	0.139	0.103
	0.155
0ng/mL	0.036	0.036	0
	0.035

Gene ID	79019
UniProt ID	Q9NSP4

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106394	E5031Hu	48T	$320		Add to Cart
BHE12106394	E5031Hu	96T	$458		Add to Cart

Human Centromere Protein M - CENPM ELISA Kit

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