Human Centromere Protein M - CENPM ELISA Kit

Mfr.No.

E5031Hu

Alternative Names

CENPM;CENP-M;Centromere protein M;Interphase centromere complex protein 39;Proliferation-associated nuclear element protein 1

Reactivity

Human

Detection Range

0.3-90ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

19737

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.17ng/mL

Background

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres.

Gene ID	79019
UniProt ID	Q9NSP4
Antigen	CENPM

Research Area

Epigenetics and Nuclear Signaling

Protocol

These standard curves of E5031Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.048	2.063	2.028
	2.078
24ng/mL	0.984	1.007	0.971
	1.03
12ng/mL	0.385	0.444	0.409
	0.503
6ng/mL	0.317	0.298	0.262
	0.278
3ng/mL	0.122	0.139	0.103
	0.155
0ng/mL	0.036	0.036	0
	0.035

Precision

We measured random samples of E5031Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	24.2	1.18	4.9
Intra-Assay	2	18	36.3	0.85	2.3
Intra-Assay	3	18	9.7	0.48	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106394	E5031Hu	48T	$320		Add to Cart
BHE12106394	E5031Hu	96T	$458		Add to Cart

Human Centromere Protein M - CENPM ELISA Kit

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