Human Centromere protein K(CENPK) ELISA kit

Human Centromere protein K(CENPK) ELISA kit

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Mfr.No.

CSB-EL005214HU

Alternative Names

centromere protein K, AF5alpha, CENP-K, FKSG14, P33, Solt, OTTHUMP00000161888, SoxLZ/Sox6 leucine zipper binding protein, SoxLZ/Sox6-binding protein Solt, leucine zipper protein FKSG14

Reactivity

Human

Detection Range

23.5 pg/mL-1500 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human OMG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	107	102-112

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human OMG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-89
1:2	Average %	97
	Range %	92-102
1:4	Average %	92
	Range %	87-98
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.645	2.534	2.590	2.452
5	1.987	2.096	2.042	1.904
2.5	1.238	1.345	1.292	1.154
1.25	0.754	0.765	0.760	0.622
0.625	0.429	0.446	0.438	0.300
0.312	0.312	0.324	0.318	0.180
0.156	0.221	0.226	0.224	0.086
0	0.137	0.138	0.138

Species

Homo sapiens

Sensitivity

5.8 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9BS16
Antigen	centromere protein K

Applications

ELISA

Research Area

CENPK

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501748	CSB-EL005214HU	96T	$695	Add to Cart
BHE10501748	CSB-EL005214HU	5x96T	$3336	Add to Cart
BHE10501748	CSB-EL005214HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.