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Home / ELISA Kit

Human Cell cycle control protein 50A (TMEM30A) ELISA Kit

SKU : BHE13702498

Antitumor alkylphospholipids initiate apoptosis in transformed HL-60 and Jurkat cells while sparing their progenitors. 1-O-Alkyl-2-carboxymethyl-sn-glycero-3-phosphocholine (Edelfosine) like other short-chained phospholipids--inflammatory platelet-activating factor (PAF) and apoptotic oxidatively truncated phospholipids--are proposed to have intracellular sites of action, yet a conduit for these choline phospholipids into mammalian cells is undefined. Edelfosine is also accumulated by Saccharomyces cerevisiae in a process requiring the membrane protein Lem3p, and the human genome contains a Lem3p homolog TMEM30a. Import of choline phospholipids into S. cerevisiae deltaLem3 is partially reconstituted by human TMEM30a and by Lem3p-TMEM30a chimeras, showing the proteins are orthologous.

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Mfr.No.	KTE60280
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Cell cycle control protein 50A (TMEM30A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM30A.
Alternative Names	TMEM30A; C6orf67; CDC50A; FLJ10856;
Detection Method	Colorimetric
Kit Components	Human Cell cycle control protein 50A microplate Human Cell cycle control protein 50A standard Human Cell cycle control protein 50A detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Cell cycle control protein 50A (TMEM30A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM30A. No significant cross-reactivity or interference between Human TMEM30A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Antitumor alkylphospholipids initiate apoptosis in transformed HL-60 and Jurkat cells while sparing their progenitors. 1-O-Alkyl-2-carboxymethyl-sn-glycero-3-phosphocholine (Edelfosine) like other short-chained phospholipids--inflammatory platelet-activating factor (PAF) and apoptotic oxidatively truncated phospholipids--are proposed to have intracellular sites of action, yet a conduit for these choline phospholipids into mammalian cells is undefined. Edelfosine is also accumulated by Saccharomyces cerevisiae in a process requiring the membrane protein Lem3p, and the human genome contains a Lem3p homolog TMEM30a. Import of choline phospholipids into S. cerevisiae deltaLem3 is partially reconstituted by human TMEM30a and by Lem3p-TMEM30a chimeras, showing the proteins are orthologous.
Notes	This Human Cell cycle control protein 50A (TMEM30A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM30A in samples. An antibody specific for TMEM30A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM30A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM30A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM30A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	55754

Applications	ELISA
Applications Notes	This Human Cell cycle control protein 50A (TMEM30A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM30A in samples. An antibody specific for TMEM30A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM30A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM30A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM30A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702498	KTE60280	48 T	$339		Add to Cart
BHE13702498	KTE60280	96 T	$589		Add to Cart
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BHE13702498	KTE60280	96 Tx50	$24569		Add to Cart

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