Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine®

Mfr.No.

EK0904

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.011	0.056	0.111	0.210	0.381	0.713	1.296	1.967

Description

Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine® (96 Tests). Quantitate Human CEACAM5 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Carcinoembryonic antigen-related cell adhesion molecule 5;Carcinoembryonic antigen;CEA;Meconium antigen 100;CD66e;CEACAM5;CEA;

Molecular Weight

76795 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: K35-A685

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human CEA

Subcellular Localization

Cell membrane ; Lipid-anchor, GPI-anchor .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	776	1668	10176	724	1662	10501
Standard deviation	39.57	124	407.04	39.09	137.94	420.04
CV (%)	4%	7.5%	4%	5.4%	8.3%	4%

Protein Function

Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. .

Background

Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	776	816	775	762	782	20.25	2.5%
Sample 2	1668	1752	1723	1646	1697	42.25	2.4%
Sample 3	10176	9323	9418	9597	9628	331.05	3.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	111518
Gene Name	CEACAM5
UniProt ID	P06731
Protein Name	Carcinoembryonic antigen-related cell adhesion molecule 5
Tissue Specificity	Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Sequence	Belongs to the immunoglobulin superfamily. CEA family.

Applications	ELISA
Research Area	Tumor Suppressors,Tumor-Associated Antigens

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000685	EK0904	96 wells/kit	$499		Add to Cart

Human CEA / Carcino Embryonic Antigen ELISA Kit PicoKine®

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